Class PdbxEntitySrcGenProteolysis

  • All Implemented Interfaces:
    Category

    @Generated("org.rcsb.cif.generator.SchemaGenerator")
    public class PdbxEntitySrcGenProteolysis
    extends BaseCategory
    This category contains details for the protein purification tag removal steps used in the overall protein production process
    • Constructor Detail

      • PdbxEntitySrcGenProteolysis

        public PdbxEntitySrcGenProteolysis​(String name,
                                           Map<String,​Column> columns)
      • PdbxEntitySrcGenProteolysis

        public PdbxEntitySrcGenProteolysis​(String name,
                                           int rowCount,
                                           Object[] encodedColumns)
      • PdbxEntitySrcGenProteolysis

        public PdbxEntitySrcGenProteolysis​(String name)
    • Method Detail

      • getEntryId

        public StrColumn getEntryId()
        The value of _pdbx_entity_src_gen_proteolysis.entry_id uniquely identifies a sample consisting of one or more proteins whose structure is to be determined. This is a pointer to _entry.id. This item may be a site dependent bar code.
        Returns:
        StrColumn
      • getEntityId

        public StrColumn getEntityId()
        The value of _pdbx_entity_src_gen_proteolysis.entity_id uniquely identifies each protein contained in the project target complex proteins whose structure is to be determined. This data item is a pointer to _entity.id in the ENTITY category. This item may be a site dependent bar code.
        Returns:
        StrColumn
      • getStepId

        public IntColumn getStepId()
        This item is the unique identifier for this tag removal step.
        Returns:
        IntColumn
      • getNextStepId

        public IntColumn getNextStepId()
        This item unique identifier for the next production step. This allows a workflow to have multiple entry points leading to a single product.
        Returns:
        IntColumn
      • getEndConstructId

        public StrColumn getEndConstructId()
        This item is a pointer to pdbx_construct.id in the PDBX_CONSTRUCT category. The referenced sequence is expected to be the amino acid sequence of the expressed product after the proteolysis step.
        Returns:
        StrColumn
      • getRobotId

        public StrColumn getRobotId()
        This data item is a pointer to pdbx_robot_system.id in the PDBX_ROBOT_SYSTEM category.
        Returns:
        StrColumn
      • getDate

        public StrColumn getDate()
        The date of production step.
        Returns:
        StrColumn
      • getDetails

        public StrColumn getDetails()
        Details of this tag removal step.
        Returns:
        StrColumn
      • getProtease

        public StrColumn getProtease()
        The name of the protease used for cleavage.
        Returns:
        StrColumn
      • getProteinProteaseRatio

        public FloatColumn getProteinProteaseRatio()
        The ratio of protein to protease used for the cleavage. = mol protein / mol protease
        Returns:
        FloatColumn
      • getCleavageBufferId

        public StrColumn getCleavageBufferId()
        This item is a pointer to pdbx_buffer.id in the PDBX_BUFFER category. The referenced buffer is that in which the cleavage was performed.
        Returns:
        StrColumn
      • getCleavageTemperature

        public FloatColumn getCleavageTemperature()
        The temperature in degrees celsius at which the cleavage was performed.
        Returns:
        FloatColumn
      • getCleavageTime

        public FloatColumn getCleavageTime()
        The time in minutes for the cleavage reaction
        Returns:
        FloatColumn