Package org.rcsb.cif.model.generated
Class PdbxEntitySrcGenExpress
- java.lang.Object
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- org.rcsb.cif.model.BaseCategory
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- org.rcsb.cif.model.generated.PdbxEntitySrcGenExpress
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- All Implemented Interfaces:
Category
@Generated("org.rcsb.cif.generator.SchemaGenerator") public class PdbxEntitySrcGenExpress extends BaseCategory
This category contains details for the EXPRESSION steps used in the overall protein production process. It is hoped that this category will cover all forms of cell-based expression by reading induction as induction/transformation/transfection.
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Field Summary
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Fields inherited from class org.rcsb.cif.model.BaseCategory
isText, textFields
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Constructor Summary
Constructors Constructor Description PdbxEntitySrcGenExpress(String name)PdbxEntitySrcGenExpress(String name, int rowCount, Object[] encodedColumns)PdbxEntitySrcGenExpress(String name, Map<String,Column> columns)
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Method Summary
All Methods Instance Methods Concrete Methods Modifier and Type Method Description StrColumngetCTerminalSeqTag()Any C-terminal sequence tag as a string of one letter amino acid codesStrColumngetCultureAdditives()Any additives to the base media in which the expression host was grown.StrColumngetCultureBaseMedia()The name of the base media in which the expression host was grown.FloatColumngetCultureTemperature()The temperature in degrees celsius at which the expression host was allowed to grow prior to induction/transformation/transfection.FloatColumngetCultureTime()The time in hours for which the expression host was allowed to grow prior to induction/transformation/transfection.FloatColumngetCultureVolume()The volume of media in milliliters in which the expression host was grown.StrColumngetDate()The date of production step.StrColumngetEndConstructId()This item is a pointer to pdbx_construct.id in the PDBX_CONSTRUCT category.StrColumngetEntityId()The value of _pdbx_entity_src_gen_express.entity_id uniquely identifies each protein contained in the project target complex proteins whose structure is to be determined.StrColumngetEntryId()The value of _pdbx_entity_src_gen_express.entry_id uniquely identifies a sample consisting of one or more proteins whose structure is to be determined.StrColumngetHarvestingDetails()Details of the harvesting protocol.StrColumngetHostOrgCellLine()A specific line of cells used as the expression systemStrColumngetHostOrgCommonName()The common name of the organism that served as host for the expression system.StrColumngetHostOrgCultureCollection()Culture collection of the expression systemStrColumngetHostOrgDetails()A description of special aspects of the organism that served as host for the expression system.StrColumngetHostOrgScientificName()The scientific name of the organism that served as host for the expression system.StrColumngetHostOrgStrain()The strain of the organism that served as host for the expression system.StrColumngetHostOrgTaxId()The id for the NCBI taxonomy node corresponding to the organism that served as host for the expression system.StrColumngetHostOrgTissue()The specific tissue which expressed the molecule.StrColumngetHostOrgVariant()The vairant of the organism that served as host for the expression system.StrColumngetInducer()The chemical name of the inducing agent.FloatColumngetInducerConcentration()Concentration of the inducing agent.StrColumngetInductionDetails()Details of induction/transformation/transfection.FloatColumngetInductionTemperature()The temperature in celsius at which the induced/transformed/transfected cells were grown.FloatColumngetInductionTimepoint()The time in hours after induction/transformation/transfection at which the optical density of the culture was measured.FloatColumngetMultiplicityOfInfection()The multiplicity of infection for genes introduced by transfection, eg.IntColumngetNextStepId()This item unique identifier for the next production step.StrColumngetNTerminalSeqTag()Any N-terminal sequence tag as a string of one letter amino acid codes.StrColumngetPlasmidId()This item is a pointer to _pdbx_construct.id in the PDBX_CONSTRUCT category.StrColumngetPromoterType()The nature of the promoter controlling expression of the gene.StrColumngetRobotId()This data item is a pointer to pdbx_robot_system.id in the PDBX_ROBOT_SYSTEM category.IntColumngetStepId()This item is the unique identifier for this expression step.StrColumngetStorageDetails()Details of how the harvested culture was stored.StrColumngetSummary()Summary of the details of the expression steps used in protein production.StrColumngetVectorType()Identifies the type of vector used (plasmid, virus, or cosmid) in the expression system.-
Methods inherited from class org.rcsb.cif.model.BaseCategory
getBinaryColumn, getCategoryName, getColumn, getColumnNames, getRowCount, isDefined
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Methods inherited from class java.lang.Object
clone, equals, finalize, getClass, hashCode, notify, notifyAll, toString, wait, wait, wait
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Methods inherited from interface org.rcsb.cif.model.Category
columnNames, columns, get
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Method Detail
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getEntryId
public StrColumn getEntryId()
The value of _pdbx_entity_src_gen_express.entry_id uniquely identifies a sample consisting of one or more proteins whose structure is to be determined. This is a pointer to _entry.id. This item may be a site dependent bar code.- Returns:
- StrColumn
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getEntityId
public StrColumn getEntityId()
The value of _pdbx_entity_src_gen_express.entity_id uniquely identifies each protein contained in the project target complex proteins whose structure is to be determined. This data item is a pointer to _entity.id in the ENTITY category. This item may be a site dependent bar code.- Returns:
- StrColumn
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getStepId
public IntColumn getStepId()
This item is the unique identifier for this expression step.- Returns:
- IntColumn
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getNextStepId
public IntColumn getNextStepId()
This item unique identifier for the next production step. This allows a workflow to have multiple entry points leading to a single product.- Returns:
- IntColumn
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getEndConstructId
public StrColumn getEndConstructId()
This item is a pointer to pdbx_construct.id in the PDBX_CONSTRUCT category. The referenced sequence is expected to be the amino acid sequence of the expressed product.- Returns:
- StrColumn
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getRobotId
public StrColumn getRobotId()
This data item is a pointer to pdbx_robot_system.id in the PDBX_ROBOT_SYSTEM category.- Returns:
- StrColumn
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getDate
public StrColumn getDate()
The date of production step.- Returns:
- StrColumn
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getPromoterType
public StrColumn getPromoterType()
The nature of the promoter controlling expression of the gene.- Returns:
- StrColumn
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getPlasmidId
public StrColumn getPlasmidId()
This item is a pointer to _pdbx_construct.id in the PDBX_CONSTRUCT category. The referenced entry will contain the nucleotide sequence that is to be expressed, including tags.- Returns:
- StrColumn
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getVectorType
public StrColumn getVectorType()
Identifies the type of vector used (plasmid, virus, or cosmid) in the expression system.- Returns:
- StrColumn
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getNTerminalSeqTag
public StrColumn getNTerminalSeqTag()
Any N-terminal sequence tag as a string of one letter amino acid codes.- Returns:
- StrColumn
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getCTerminalSeqTag
public StrColumn getCTerminalSeqTag()
Any C-terminal sequence tag as a string of one letter amino acid codes- Returns:
- StrColumn
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getHostOrgScientificName
public StrColumn getHostOrgScientificName()
The scientific name of the organism that served as host for the expression system. It is expected that either this item or _pdbx_entity_src_gen_express.host_org_tax_id should be populated.- Returns:
- StrColumn
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getHostOrgCommonName
public StrColumn getHostOrgCommonName()
The common name of the organism that served as host for the expression system. Where _pdbx_entity_src_gen_express.host_org_tax_id is populated it is expected that this item may be derived by look up against the taxonomy database.- Returns:
- StrColumn
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getHostOrgVariant
public StrColumn getHostOrgVariant()
The vairant of the organism that served as host for the expression system. Where _pdbx_entity_src_gen_express.host_org_tax_id is populated it is expected that this item may be derived by a look up against the taxonomy database.- Returns:
- StrColumn
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getHostOrgStrain
public StrColumn getHostOrgStrain()
The strain of the organism that served as host for the expression system. Where _pdbx_entity_src_gen_express.host_org_tax_id is populated it is expected that this item may be derived by a look up against the taxonomy database.- Returns:
- StrColumn
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getHostOrgTissue
public StrColumn getHostOrgTissue()
The specific tissue which expressed the molecule.- Returns:
- StrColumn
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getHostOrgCultureCollection
public StrColumn getHostOrgCultureCollection()
Culture collection of the expression system- Returns:
- StrColumn
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getHostOrgCellLine
public StrColumn getHostOrgCellLine()
A specific line of cells used as the expression system- Returns:
- StrColumn
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getHostOrgTaxId
public StrColumn getHostOrgTaxId()
The id for the NCBI taxonomy node corresponding to the organism that served as host for the expression system.- Returns:
- StrColumn
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getHostOrgDetails
public StrColumn getHostOrgDetails()
A description of special aspects of the organism that served as host for the expression system.- Returns:
- StrColumn
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getCultureBaseMedia
public StrColumn getCultureBaseMedia()
The name of the base media in which the expression host was grown.- Returns:
- StrColumn
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getCultureAdditives
public StrColumn getCultureAdditives()
Any additives to the base media in which the expression host was grown.- Returns:
- StrColumn
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getCultureVolume
public FloatColumn getCultureVolume()
The volume of media in milliliters in which the expression host was grown.- Returns:
- FloatColumn
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getCultureTime
public FloatColumn getCultureTime()
The time in hours for which the expression host was allowed to grow prior to induction/transformation/transfection.- Returns:
- FloatColumn
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getCultureTemperature
public FloatColumn getCultureTemperature()
The temperature in degrees celsius at which the expression host was allowed to grow prior to induction/transformation/transfection.- Returns:
- FloatColumn
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getInducer
public StrColumn getInducer()
The chemical name of the inducing agent.- Returns:
- StrColumn
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getInducerConcentration
public FloatColumn getInducerConcentration()
Concentration of the inducing agent.- Returns:
- FloatColumn
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getInductionDetails
public StrColumn getInductionDetails()
Details of induction/transformation/transfection.- Returns:
- StrColumn
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getMultiplicityOfInfection
public FloatColumn getMultiplicityOfInfection()
The multiplicity of infection for genes introduced by transfection, eg. for baculovirus-based expression.- Returns:
- FloatColumn
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getInductionTimepoint
public FloatColumn getInductionTimepoint()
The time in hours after induction/transformation/transfection at which the optical density of the culture was measured.- Returns:
- FloatColumn
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getInductionTemperature
public FloatColumn getInductionTemperature()
The temperature in celsius at which the induced/transformed/transfected cells were grown.- Returns:
- FloatColumn
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getHarvestingDetails
public StrColumn getHarvestingDetails()
Details of the harvesting protocol.- Returns:
- StrColumn
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getStorageDetails
public StrColumn getStorageDetails()
Details of how the harvested culture was stored.- Returns:
- StrColumn
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getSummary
public StrColumn getSummary()
Summary of the details of the expression steps used in protein production.- Returns:
- StrColumn
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