Class PdbxEntitySrcGenCloneLigation

  • All Implemented Interfaces:
    Category

    @Generated("org.rcsb.cif.generator.SchemaGenerator")
    public class PdbxEntitySrcGenCloneLigation
    extends BaseCategory
    This category contains details for the ligation-based cloning steps used in the overall protein production process. _pdbx_entity_src_gen_clone_ligation.clone_step_id in this category must point at a defined _pdbx_entity_src_gen_clone.step_id. The details in PDBX_ENTITY_SRC_GEN_CLONE_LIGATION extend the details in PDBX_ENTITY_SRC_GEN_CLONE to cover ligation dependent cloning steps.
    • Constructor Detail

      • PdbxEntitySrcGenCloneLigation

        public PdbxEntitySrcGenCloneLigation​(String name,
                                             Map<String,​Column> columns)
      • PdbxEntitySrcGenCloneLigation

        public PdbxEntitySrcGenCloneLigation​(String name,
                                             int rowCount,
                                             Object[] encodedColumns)
      • PdbxEntitySrcGenCloneLigation

        public PdbxEntitySrcGenCloneLigation​(String name)
    • Method Detail

      • getEntryId

        public StrColumn getEntryId()
        This item is a pointer to _pdbx_entity_src_gen_clone.entry_id in the PDBX_ENTITY_SRC_GEN_CLONE category.
        Returns:
        StrColumn
      • getEntityId

        public StrColumn getEntityId()
        This item is a pointer to _pdbx_entity_src_gen_clone.entity_id in the PDBX_ENTITY_SRC_GEN_CLONE category.
        Returns:
        StrColumn
      • getStepId

        public IntColumn getStepId()
        This item is a pointer to _pdbx_entity_src_gen_clone.step_id in the PDBX_ENTITY_SRC_GEN_CLONE category.
        Returns:
        IntColumn
      • getCleavageEnzymes

        public StrColumn getCleavageEnzymes()
        The names of the enzymes used to cleave the vector. In addition an enzyme used to blunt the cut ends, etc., should be named here.
        Returns:
        StrColumn
      • getLigationEnzymes

        public StrColumn getLigationEnzymes()
        The names of the enzymes used to ligate the gene into the cleaved vector.
        Returns:
        StrColumn
      • getTemperature

        public FloatColumn getTemperature()
        The temperature at which the ligation experiment was performed, in degrees celsius.
        Returns:
        FloatColumn
      • getTime

        public IntColumn getTime()
        The duration of the ligation reaction in minutes.
        Returns:
        IntColumn
      • getDetails

        public StrColumn getDetails()
        Any details to be associated with this ligation step, e.g. the protocol.
        Returns:
        StrColumn