Class PdbxEntitySrcGenClone

  • All Implemented Interfaces:
    Category

    @Generated("org.rcsb.cif.generator.SchemaGenerator")
    public class PdbxEntitySrcGenClone
    extends BaseCategory
    This category contains details for the cloning steps used in the overall protein production process. Each row in PDBX_ENTITY_SRC_GEN_CLONE should have an equivalent row in either PDBX_ENTITY_SRC_GEN_CLONE_LIGATION or PDBX_ENTITY_SRC_GEN_CLONE_RECOMBINATION. If only summary information is provided data in the later two categories may be omitted.
    • Constructor Detail

      • PdbxEntitySrcGenClone

        public PdbxEntitySrcGenClone​(String name,
                                     int rowCount,
                                     Object[] encodedColumns)
      • PdbxEntitySrcGenClone

        public PdbxEntitySrcGenClone​(String name)
    • Method Detail

      • getEntryId

        public StrColumn getEntryId()
        The value of _pdbx_entity_src_gen_clone.entry_id uniquely identifies a sample consisting of one or more proteins whose structure is to be determined. This is a pointer to _entry.id. This item may be a site dependent bar code.
        Returns:
        StrColumn
      • getEntityId

        public StrColumn getEntityId()
        The value of _pdbx_entity_src_gen_clone.entity_id uniquely identifies each protein contained in the project target protein complex whose structure is to be determined. This data item is a pointer to _entity.id in the ENTITY category. This item may be a site dependent bar code.
        Returns:
        StrColumn
      • getStepId

        public IntColumn getStepId()
        This item is the unique identifier for this cloning step.
        Returns:
        IntColumn
      • getNextStepId

        public IntColumn getNextStepId()
        This item unique identifier for the next production step. This allows a workflow to have multiple entry points leading to a single product.
        Returns:
        IntColumn
      • getEndConstructId

        public StrColumn getEndConstructId()
        This item is a pointer to pdbx_construct.id in the PDBX_CONSTRUCT category. The referenced nucleic acid sequence is that of the cloned product.
        Returns:
        StrColumn
      • getRobotId

        public StrColumn getRobotId()
        This data item is a pointer to pdbx_robot_system.id in the PDBX_ROBOT_SYSTEM category.
        Returns:
        StrColumn
      • getDate

        public StrColumn getDate()
        The date of this production step.
        Returns:
        StrColumn
      • getGeneInsertMethod

        public StrColumn getGeneInsertMethod()
        The method used to insert the gene into the vector. For 'Ligation', an PDBX_ENTITY_SRC_GEN_CLONE_LIGATION entry with matching .step_id is expected. For 'Recombination', an PDBX_ENTITY_SRC_GEN_CLONE_RECOMBINATION entry with matching .step_id is expected.
        Returns:
        StrColumn
      • getVectorName

        public StrColumn getVectorName()
        The name of the vector used in this cloning step.
        Returns:
        StrColumn
      • getVectorDetails

        public StrColumn getVectorDetails()
        Details of any modifications made to the named vector.
        Returns:
        StrColumn
      • getTransformationMethod

        public StrColumn getTransformationMethod()
        The method used to transform the expression cell line with the vector
        Returns:
        StrColumn
      • getMarker

        public StrColumn getMarker()
        The type of marker included to allow selection of transformed cells
        Returns:
        StrColumn
      • getVerificationMethod

        public StrColumn getVerificationMethod()
        The method used to verify that the incorporated gene is correct
        Returns:
        StrColumn
      • getPurificationDetails

        public StrColumn getPurificationDetails()
        Details of any purification of the product.
        Returns:
        StrColumn
      • getSummary

        public StrColumn getSummary()
        Summary of ligation or recombionation cloning used, the associated verification method and any purification of the product.
        Returns:
        StrColumn