htsjdk.samtools

Class SamReaderFactory

java.lang.Object

htsjdk.samtools.SamReaderFactory

public abstract class SamReaderFactory
extends java.lang.Object

Describes the functionality for producing SamReader, and offers a handful of static generators.

     SamReaderFactory.makeDefault().open(new File("/my/bam.bam");
 

Example: Configure a factory

      final SamReaderFactory factory =
          SamReaderFactory.makeDefault()
              .enable(SamReaderFactory.Option.INCLUDE_SOURCE_IN_RECORDS, SamReaderFactory.Option.VALIDATE_CRC_CHECKSUMS)
              .validationStringency(ValidationStringency.SILENT);

 

Example: Open two bam files from different sources, using different options

     final SamReaderFactory factory =
          SamReaderFactory.makeDefault()
              .enable(SamReaderFactory.Option.INCLUDE_SOURCE_IN_RECORDS, SamReaderFactory.Option.VALIDATE_CRC_CHECKSUMS)
              .validationStringency(ValidationStringency.SILENT);

     // File-based bam
     final SamReader fileReader = factory.open(new File("/my/bam.bam"));

     // HTTP-hosted BAM with index from an arbitrary stream
     final SeekableStream myBamIndexStream = ...
     final SamInputResource resource =
          SamInputResource.of(new URL("http://example.com/data.bam")).index(myBamIndexStream);
     final SamReader complicatedReader = factory.open(resource);
 

Nested Class Summary

Nested Classes

Modifier and Type	Class and Description
static class	SamReaderFactory.Option A collection of binary SamReaderFactory options.

Constructor Summary

Constructors

Constructor and Description
SamReaderFactory()

Method Summary

Methods

Modifier and Type	Method and Description
abstract SamReaderFactory	disable(SamReaderFactory.Option... options) Disables the provided SamReaderFactory.Options, then returns itself.
abstract SamReaderFactory	enable(SamReaderFactory.Option... options) Enables the provided SamReaderFactory.Options, then returns itself.
abstract SAMFileHeader	getFileHeader(java.io.File samFile) Utility method to open the file get the header and close the file
static SamReaderFactory	make() Creates an "empty" factory with no enabled SamReaderFactory.Options, ValidationStringency.DEFAULT_STRINGENCY, and DefaultSAMRecordFactory.
static SamReaderFactory	makeDefault() Creates a copy of the default SamReaderFactory.
abstract SamReader	open(java.io.File file)
abstract SamReader	open(SamInputResource resource)
abstract void	reapplyOptions(SamReader reader) Reapplies any changed options to the reader *
abstract SamReaderFactory	referenceSequence(java.io.File referenceSequence) Sets the specified reference sequence *
abstract ReferenceSource	referenceSource()
abstract SamReaderFactory	referenceSource(ReferenceSource referenceSequence) Sets the specified reference sequence *
abstract SamReaderFactory	samRecordFactory(SAMRecordFactory samRecordFactory) Set this factory's SAMRecordFactory to the provided one, then returns itself.
static void	setDefaultValidationStringency(ValidationStringency defaultValidationStringency)
abstract SamReaderFactory	setOption(SamReaderFactory.Option option, boolean value) Sets a specific Option to a boolean value.
abstract ValidationStringency	validationStringency()
abstract SamReaderFactory	validationStringency(ValidationStringency validationStringency) Set this factory's ValidationStringency to the provided one, then returns itself.

Methods inherited from class java.lang.Object

clone, equals, finalize, getClass, hashCode, notify, notifyAll, toString, wait, wait, wait

Constructor Detail

SamReaderFactory

public SamReaderFactory()

Method Detail

open

public abstract SamReader open(java.io.File file)

open

public abstract SamReader open(SamInputResource resource)

validationStringency

public abstract ValidationStringency validationStringency()

referenceSource

public abstract ReferenceSource referenceSource()

samRecordFactory

public abstract SamReaderFactory samRecordFactory(SAMRecordFactory samRecordFactory)

Set this factory's SAMRecordFactory to the provided one, then returns itself.

enable

public abstract SamReaderFactory enable(SamReaderFactory.Option... options)

Enables the provided SamReaderFactory.Options, then returns itself.

disable

public abstract SamReaderFactory disable(SamReaderFactory.Option... options)

Disables the provided SamReaderFactory.Options, then returns itself.

setOption

public abstract SamReaderFactory setOption(SamReaderFactory.Option option,
                         boolean value)

Sets a specific Option to a boolean value. *

referenceSequence

public abstract SamReaderFactory referenceSequence(java.io.File referenceSequence)

Sets the specified reference sequence *

referenceSource

public abstract SamReaderFactory referenceSource(ReferenceSource referenceSequence)

Sets the specified reference sequence *

getFileHeader

public abstract SAMFileHeader getFileHeader(java.io.File samFile)

Utility method to open the file get the header and close the file

reapplyOptions

public abstract void reapplyOptions(SamReader reader)

Reapplies any changed options to the reader *

validationStringency

public abstract SamReaderFactory validationStringency(ValidationStringency validationStringency)

Set this factory's ValidationStringency to the provided one, then returns itself.

setDefaultValidationStringency

public static void setDefaultValidationStringency(ValidationStringency defaultValidationStringency)

makeDefault

public static SamReaderFactory makeDefault()

Creates a copy of the default SamReaderFactory.

make

public static SamReaderFactory make()

Creates an "empty" factory with no enabled SamReaderFactory.Options, ValidationStringency.DEFAULT_STRINGENCY, and DefaultSAMRecordFactory.